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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A research laboratory is investigating the mechanism of action of a novel antiviral compound. In vitro studies demonstrate that the compound effectively inhibits the replication of a specific enveloped RNA virus. Further analysis reveals that the compound does not interfere with viral entry, genome replication, or protein synthesis. However, electron microscopy of infected cells treated with the compound shows an accumulation of viral glycoproteins within the endoplasmic reticulum and Golgi apparatus, with a significant reduction in the release of infectious virions. The MOST likely target of this antiviral compound is:

A) Viral glycoprotein trafficking and maturation
B) Viral RNA-dependent RNA polymerase
C) Viral protease responsible for polyprotein cleavage
D) Host cell membrane fusion machinery

2. A centrifuge rotor containing capped tubes of sputum concentrate for AFB culture breaks during operation inside a Class II Biological Safety Cabinet (BSC). What is the most appropriate immediate action for the technologist?

A) Immediately turn off the BSC, open the sash fully, and spray disinfectant widely.
B) Keep the BSC running, notify the supervisor, evacuate the immediate area, and post warning signs.
Allow aerosols to settle (e.g., 30 min) before initiating decontamination with a tuberculocidal disinfectant.
C) Open the centrifuge within the BSC, discard broken glass in a regular sharps container, and clean the spill with 10% bleach.
D) Carefully remove any intact tubes, wipe them with 70% alcohol, and place them in a new rotor to continue processing.

3. A microbiology laboratory is processing a sputum sample from a patient with chronic cough and night sweats.
After Ziehl-Neelsen staining, acid-fast bacilli are observed. Culture on Lowenstein-Jensen medium is positive after several weeks. Drug susceptibility testing reveals resistance to isoniazid and rifampin. This patient is MOST likely infected with:

A) Multidrug-resistant Mycobacterium tuberculosis
B) Nocardia asteroides
C) Mycobacterium avium complex
D) Actinomyces israelii

4. During the processing of stool specimens for ova and parasite examination, the formalin-ethyl acetate concentration procedure primarily serves to:

A) Separate parasites from fecal debris and concentrate them
B) Selectively culture protozoan trophozoites
C) Stain parasite nuclei for easier identification
D) Preserve parasite morphology and kill bacteria

5. A patient with a history of travel to South America presents with fever, lymphadenopathy, and hepatosplenomegaly. Peripheral blood smear reveals the presence of trypomastigotes. The MOST likely diagnosis is:

A) Leishmaniasis
B) Chagas disease (American trypanosomiasis)
C) Malaria
D) African trypanosomiasis (sleeping sickness)

Solutions: